Monday, March 7, 2011

vector cloning vs PCR

vector cloning
-slower than PCR
-requires restriction enzymes, ligase, and vector DNA
-uses plasmid
-injection of plasmid into bacterial cell
-proteins can be produced

PCR
-faster than vector cloning
-used to amplify a target sequence
-only requires a single copy of DNA
-can be done with a machine
-forensic uses
-If the first copy is contaminated, the rest would also be contaminated.

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